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rat tlr4 elisa kit  (Cusabio)


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    Cusabio rat tlr4 elisa kit
    Tongue tissue <t>TLR4</t> and proinflammatory cytokines. A TLR4 (ng/mg protein), ( B ) TNF-α (ng/L/g), ( C ) IL-6 (ng/L/g) (mean ± SEM). DOX increased TLR4/TNF-α/IL-6; EDO reduced these levels. Statistics: TLR4/IL-6—ANOVA ± Tukey; TNF-α—Kruskal–Wallis ± Mann–Whitney. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant (adjusted p ≥ 0.05)
    Rat Tlr4 Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat tlr4 elisa kit/product/Cusabio
    Average 93 stars, based on 21 article reviews
    rat tlr4 elisa kit - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Edaravone attenuates doxorubicin-induced oral mucosal injury via modulation of oxidative stress, inflammatory signaling, and the SIRT1/TLR4/NF-kB/ACE2 axis in rats"

    Article Title: Edaravone attenuates doxorubicin-induced oral mucosal injury via modulation of oxidative stress, inflammatory signaling, and the SIRT1/TLR4/NF-kB/ACE2 axis in rats

    Journal: BMC Oral Health

    doi: 10.1186/s12903-025-07148-y

    Tongue tissue TLR4 and proinflammatory cytokines. A TLR4 (ng/mg protein), ( B ) TNF-α (ng/L/g), ( C ) IL-6 (ng/L/g) (mean ± SEM). DOX increased TLR4/TNF-α/IL-6; EDO reduced these levels. Statistics: TLR4/IL-6—ANOVA ± Tukey; TNF-α—Kruskal–Wallis ± Mann–Whitney. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant (adjusted p ≥ 0.05)
    Figure Legend Snippet: Tongue tissue TLR4 and proinflammatory cytokines. A TLR4 (ng/mg protein), ( B ) TNF-α (ng/L/g), ( C ) IL-6 (ng/L/g) (mean ± SEM). DOX increased TLR4/TNF-α/IL-6; EDO reduced these levels. Statistics: TLR4/IL-6—ANOVA ± Tukey; TNF-α—Kruskal–Wallis ± Mann–Whitney. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant (adjusted p ≥ 0.05)

    Techniques Used: MANN-WHITNEY



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    Tongue tissue <t>TLR4</t> and proinflammatory cytokines. A TLR4 (ng/mg protein), ( B ) TNF-α (ng/L/g), ( C ) IL-6 (ng/L/g) (mean ± SEM). DOX increased TLR4/TNF-α/IL-6; EDO reduced these levels. Statistics: TLR4/IL-6—ANOVA ± Tukey; TNF-α—Kruskal–Wallis ± Mann–Whitney. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant (adjusted p ≥ 0.05)
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    Tongue tissue <t>TLR4</t> and proinflammatory cytokines. A TLR4 (ng/mg protein), ( B ) TNF-α (ng/L/g), ( C ) IL-6 (ng/L/g) (mean ± SEM). DOX increased TLR4/TNF-α/IL-6; EDO reduced these levels. Statistics: TLR4/IL-6—ANOVA ± Tukey; TNF-α—Kruskal–Wallis ± Mann–Whitney. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant (adjusted p ≥ 0.05)
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    Impact of GA, DEX, and combination of GA and DEX on LPS-induced changes in inflammatory markers. ( A ) Renal SIRT1 level, ( B ) renal <t>TLR4</t> level, ( C ) renal MYD88 level, ( D ) renal IL-1β level, and ( E ) renal TNF-α level. LPS: lipopolysaccharide; DEX: dexamethasone; GA: Gum acacia; SIRT1: Silent information regulator 2-related enzyme 1; TLR4: Toll-like receptor 4; TNF-α: tumor necrosis factor α; IL-1β: interleukin -1β; MYD88: myeloid differentiation primary response 88. GA administration started on day 1 and lasted for 14 days. LPS was injected on day 14, and DEX was injected 2 h post LPS injection. Data were expressed as means ± S.E.M. (n = 6 rats per group). Mean values were compared using one-way ANOVA followed by post hoc Tukey’s multiple comparison test: * p < 0.05, vs. CTR group; # p < 0.05, vs. LPS group.
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    FIGURE 4 Inflammatory cytokines and NLRP3 and <t>TLR4</t> expression in the sham-operation and RIRI group. Western blot assay of lL-1β, IL-6, IL-8, NLRP3, and TLR4 in sham-operation and RIRI group using kidney tissue (A). Level of lL-1β, IL-6, IL-8, NLRP3, and TLR4 in the sham- operation and RIRI group via ELISA using plasma (B–F). Data are shown as mean ± SD, n = 3; *p < 0.05; **p < 0.01.
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    FIGURE 4 Inflammatory cytokines and NLRP3 and <t>TLR4</t> expression in the sham-operation and RIRI group. Western blot assay of lL-1β, IL-6, IL-8, NLRP3, and TLR4 in sham-operation and RIRI group using kidney tissue (A). Level of lL-1β, IL-6, IL-8, NLRP3, and TLR4 in the sham- operation and RIRI group via ELISA using plasma (B–F). Data are shown as mean ± SD, n = 3; *p < 0.05; **p < 0.01.
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    Image Search Results


    Tongue tissue TLR4 and proinflammatory cytokines. A TLR4 (ng/mg protein), ( B ) TNF-α (ng/L/g), ( C ) IL-6 (ng/L/g) (mean ± SEM). DOX increased TLR4/TNF-α/IL-6; EDO reduced these levels. Statistics: TLR4/IL-6—ANOVA ± Tukey; TNF-α—Kruskal–Wallis ± Mann–Whitney. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant (adjusted p ≥ 0.05)

    Journal: BMC Oral Health

    Article Title: Edaravone attenuates doxorubicin-induced oral mucosal injury via modulation of oxidative stress, inflammatory signaling, and the SIRT1/TLR4/NF-kB/ACE2 axis in rats

    doi: 10.1186/s12903-025-07148-y

    Figure Lengend Snippet: Tongue tissue TLR4 and proinflammatory cytokines. A TLR4 (ng/mg protein), ( B ) TNF-α (ng/L/g), ( C ) IL-6 (ng/L/g) (mean ± SEM). DOX increased TLR4/TNF-α/IL-6; EDO reduced these levels. Statistics: TLR4/IL-6—ANOVA ± Tukey; TNF-α—Kruskal–Wallis ± Mann–Whitney. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant (adjusted p ≥ 0.05)

    Article Snippet: TLR4 protein levels in tongue tissue were quantified using a rat TLR4 ELISA kit (Cusabio, Cat. No: CSB-E15822r).

    Techniques: MANN-WHITNEY

    Impact of GA, DEX, and combination of GA and DEX on LPS-induced changes in inflammatory markers. ( A ) Renal SIRT1 level, ( B ) renal TLR4 level, ( C ) renal MYD88 level, ( D ) renal IL-1β level, and ( E ) renal TNF-α level. LPS: lipopolysaccharide; DEX: dexamethasone; GA: Gum acacia; SIRT1: Silent information regulator 2-related enzyme 1; TLR4: Toll-like receptor 4; TNF-α: tumor necrosis factor α; IL-1β: interleukin -1β; MYD88: myeloid differentiation primary response 88. GA administration started on day 1 and lasted for 14 days. LPS was injected on day 14, and DEX was injected 2 h post LPS injection. Data were expressed as means ± S.E.M. (n = 6 rats per group). Mean values were compared using one-way ANOVA followed by post hoc Tukey’s multiple comparison test: * p < 0.05, vs. CTR group; # p < 0.05, vs. LPS group.

    Journal: Pharmaceuticals

    Article Title: Gum Acacia–Dexamethasone Combination Attenuates Sepsis-Induced Acute Kidney Injury in Rats via Targeting SIRT1-HMGB1 Signaling Pathway and Preserving Mitochondrial Integrity

    doi: 10.3390/ph18081164

    Figure Lengend Snippet: Impact of GA, DEX, and combination of GA and DEX on LPS-induced changes in inflammatory markers. ( A ) Renal SIRT1 level, ( B ) renal TLR4 level, ( C ) renal MYD88 level, ( D ) renal IL-1β level, and ( E ) renal TNF-α level. LPS: lipopolysaccharide; DEX: dexamethasone; GA: Gum acacia; SIRT1: Silent information regulator 2-related enzyme 1; TLR4: Toll-like receptor 4; TNF-α: tumor necrosis factor α; IL-1β: interleukin -1β; MYD88: myeloid differentiation primary response 88. GA administration started on day 1 and lasted for 14 days. LPS was injected on day 14, and DEX was injected 2 h post LPS injection. Data were expressed as means ± S.E.M. (n = 6 rats per group). Mean values were compared using one-way ANOVA followed by post hoc Tukey’s multiple comparison test: * p < 0.05, vs. CTR group; # p < 0.05, vs. LPS group.

    Article Snippet: Inflammation Biomarkers: Sirtuin-1 (SIRT1) (BT LABORATORY, Beijing, China, E1145Ra), tumor necrosis factor α (TNF-α) (CUSABIO, Houston, TA, USA, CSB-E11987r), Toll-like receptor 4 (TLR4) (CUSABIO, Houston, TA, USA, CSB-E15822r), myeloid differentiation primary response 88 (MYD88) (Assay Genie, Dublin, Ireland, RTFI01303), and interleukin-1β (IL-1β) (CUSABIO, Houston, TA, USA, CSB-E08055r) were quantified in kidney homogenate using ELISA kits according to the manufacturer’s instructions.

    Techniques: Injection, Comparison

    FIGURE 4 Inflammatory cytokines and NLRP3 and TLR4 expression in the sham-operation and RIRI group. Western blot assay of lL-1β, IL-6, IL-8, NLRP3, and TLR4 in sham-operation and RIRI group using kidney tissue (A). Level of lL-1β, IL-6, IL-8, NLRP3, and TLR4 in the sham- operation and RIRI group via ELISA using plasma (B–F). Data are shown as mean ± SD, n = 3; *p < 0.05; **p < 0.01.

    Journal: The Kaohsiung journal of medical sciences

    Article Title: MicroRNA-223 alleviates inflammatory response in renal ischemia-reperfusion injury by targeting NLRP3.

    doi: 10.1002/kjm2.12883

    Figure Lengend Snippet: FIGURE 4 Inflammatory cytokines and NLRP3 and TLR4 expression in the sham-operation and RIRI group. Western blot assay of lL-1β, IL-6, IL-8, NLRP3, and TLR4 in sham-operation and RIRI group using kidney tissue (A). Level of lL-1β, IL-6, IL-8, NLRP3, and TLR4 in the sham- operation and RIRI group via ELISA using plasma (B–F). Data are shown as mean ± SD, n = 3; *p < 0.05; **p < 0.01.

    Article Snippet: The detailed ELISA kits used were as follow: rat interleukin 1β (IL-1β) ELISA Kit (CUSABIO, CSB-E08055r, China), rat interleukin 6 (IL-6) ELISA Kit (CUSABIO, CSB-E04640r, China), rat IL-8 ELISA kit (ZCi Bio, ZC-36406), rat NLRP3 ELISA kit (ZCi Bio, ZC-54271, China), and rat toll-like receptor 4 (TLR4) ELISA kit (CUSABIO, CSB-E15822r, China).

    Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

    FIGURE 5 Relationship between miR-223 and the TLR4/NF-κB pathway in RIRI mice. Protein levels of TLR4 and NF-κB in the kidneys of RIRI mice at 0, 3, 6, and 12 h by western blotting (A). Quantitative analysis of NF-κB and TLR4 protein expression levels in sham and RIRI groups at 0, 3, 6, and 12 h (B). With an increase in miR- 223 expression at 6 h, the protein levels of TLR4 and NF-κB gradually decreased. Data are shown as the mean ± SD. *p < 0.05; **p < 0.01.

    Journal: The Kaohsiung journal of medical sciences

    Article Title: MicroRNA-223 alleviates inflammatory response in renal ischemia-reperfusion injury by targeting NLRP3.

    doi: 10.1002/kjm2.12883

    Figure Lengend Snippet: FIGURE 5 Relationship between miR-223 and the TLR4/NF-κB pathway in RIRI mice. Protein levels of TLR4 and NF-κB in the kidneys of RIRI mice at 0, 3, 6, and 12 h by western blotting (A). Quantitative analysis of NF-κB and TLR4 protein expression levels in sham and RIRI groups at 0, 3, 6, and 12 h (B). With an increase in miR- 223 expression at 6 h, the protein levels of TLR4 and NF-κB gradually decreased. Data are shown as the mean ± SD. *p < 0.05; **p < 0.01.

    Article Snippet: The detailed ELISA kits used were as follow: rat interleukin 1β (IL-1β) ELISA Kit (CUSABIO, CSB-E08055r, China), rat interleukin 6 (IL-6) ELISA Kit (CUSABIO, CSB-E04640r, China), rat IL-8 ELISA kit (ZCi Bio, ZC-36406), rat NLRP3 ELISA kit (ZCi Bio, ZC-54271, China), and rat toll-like receptor 4 (TLR4) ELISA kit (CUSABIO, CSB-E15822r, China).

    Techniques: Western Blot, Expressing

    FIGURE 6 Schematic diagram of miR- 223 regulating the TLR4/NF-κB pathway.

    Journal: The Kaohsiung journal of medical sciences

    Article Title: MicroRNA-223 alleviates inflammatory response in renal ischemia-reperfusion injury by targeting NLRP3.

    doi: 10.1002/kjm2.12883

    Figure Lengend Snippet: FIGURE 6 Schematic diagram of miR- 223 regulating the TLR4/NF-κB pathway.

    Article Snippet: The detailed ELISA kits used were as follow: rat interleukin 1β (IL-1β) ELISA Kit (CUSABIO, CSB-E08055r, China), rat interleukin 6 (IL-6) ELISA Kit (CUSABIO, CSB-E04640r, China), rat IL-8 ELISA kit (ZCi Bio, ZC-36406), rat NLRP3 ELISA kit (ZCi Bio, ZC-54271, China), and rat toll-like receptor 4 (TLR4) ELISA kit (CUSABIO, CSB-E15822r, China).

    Techniques:

    FIGURE 7 MicroRNA was down- regulated in H/R compared to control and H/R significantly elevated inflammatory cytokines and NLRP3 and TLR4 in HK-2 cells at the 0 h mark. RT-gPCR of miR-223 in the control and H/R group (A); western blot assay of lL-1β, IL-6, IL-8, NLRP3, and TLR4 expression level in control and H/R group (B), quantitative analysis of lL-1β, IL-6, IL-8, NLRP3, and TLR4 via western blot assay (C). Data are shown as mean ± SD, n = 3; *p < 0.05; **p < 0.01.

    Journal: The Kaohsiung journal of medical sciences

    Article Title: MicroRNA-223 alleviates inflammatory response in renal ischemia-reperfusion injury by targeting NLRP3.

    doi: 10.1002/kjm2.12883

    Figure Lengend Snippet: FIGURE 7 MicroRNA was down- regulated in H/R compared to control and H/R significantly elevated inflammatory cytokines and NLRP3 and TLR4 in HK-2 cells at the 0 h mark. RT-gPCR of miR-223 in the control and H/R group (A); western blot assay of lL-1β, IL-6, IL-8, NLRP3, and TLR4 expression level in control and H/R group (B), quantitative analysis of lL-1β, IL-6, IL-8, NLRP3, and TLR4 via western blot assay (C). Data are shown as mean ± SD, n = 3; *p < 0.05; **p < 0.01.

    Article Snippet: The detailed ELISA kits used were as follow: rat interleukin 1β (IL-1β) ELISA Kit (CUSABIO, CSB-E08055r, China), rat interleukin 6 (IL-6) ELISA Kit (CUSABIO, CSB-E04640r, China), rat IL-8 ELISA kit (ZCi Bio, ZC-36406), rat NLRP3 ELISA kit (ZCi Bio, ZC-54271, China), and rat toll-like receptor 4 (TLR4) ELISA kit (CUSABIO, CSB-E15822r, China).

    Techniques: Control, Western Blot, Expressing

    FIGURE 8 MiR-223 could alleviate inflammation in H/R. Western blot assay of IL-1β, IL-6, IL-8, NLRP3, and TLR4 in H/R and Control with or without miR-223 overexpression in HK-2 cells (A); quantitative analysis of western blot of relative protein (B–F). Data are shown as mean ± SD, n = 3; *p < 0.05; **p < 0.01.

    Journal: The Kaohsiung journal of medical sciences

    Article Title: MicroRNA-223 alleviates inflammatory response in renal ischemia-reperfusion injury by targeting NLRP3.

    doi: 10.1002/kjm2.12883

    Figure Lengend Snippet: FIGURE 8 MiR-223 could alleviate inflammation in H/R. Western blot assay of IL-1β, IL-6, IL-8, NLRP3, and TLR4 in H/R and Control with or without miR-223 overexpression in HK-2 cells (A); quantitative analysis of western blot of relative protein (B–F). Data are shown as mean ± SD, n = 3; *p < 0.05; **p < 0.01.

    Article Snippet: The detailed ELISA kits used were as follow: rat interleukin 1β (IL-1β) ELISA Kit (CUSABIO, CSB-E08055r, China), rat interleukin 6 (IL-6) ELISA Kit (CUSABIO, CSB-E04640r, China), rat IL-8 ELISA kit (ZCi Bio, ZC-36406), rat NLRP3 ELISA kit (ZCi Bio, ZC-54271, China), and rat toll-like receptor 4 (TLR4) ELISA kit (CUSABIO, CSB-E15822r, China).

    Techniques: Western Blot, Control, Over Expression

    FIGURE 11 Lock of NLRP3 could abolish the anti-inflammatory effects of miR-223. Verification of SiRNA-NLRP3 in HK-2 cells, immunofluorescence (A), western blot (B); western blot assay of IL-1β, IL-6, IL-8, NLRP3, and TLR4 in H/R, H/R + miR-223, and H/R + miR-223 + SiRNA-NLRP3 group (C), quantitative analysis of the relative protein in each group (D). Data are shown as mean ± SD.*p < 0.05; **p < 0.01.

    Journal: The Kaohsiung journal of medical sciences

    Article Title: MicroRNA-223 alleviates inflammatory response in renal ischemia-reperfusion injury by targeting NLRP3.

    doi: 10.1002/kjm2.12883

    Figure Lengend Snippet: FIGURE 11 Lock of NLRP3 could abolish the anti-inflammatory effects of miR-223. Verification of SiRNA-NLRP3 in HK-2 cells, immunofluorescence (A), western blot (B); western blot assay of IL-1β, IL-6, IL-8, NLRP3, and TLR4 in H/R, H/R + miR-223, and H/R + miR-223 + SiRNA-NLRP3 group (C), quantitative analysis of the relative protein in each group (D). Data are shown as mean ± SD.*p < 0.05; **p < 0.01.

    Article Snippet: The detailed ELISA kits used were as follow: rat interleukin 1β (IL-1β) ELISA Kit (CUSABIO, CSB-E08055r, China), rat interleukin 6 (IL-6) ELISA Kit (CUSABIO, CSB-E04640r, China), rat IL-8 ELISA kit (ZCi Bio, ZC-36406), rat NLRP3 ELISA kit (ZCi Bio, ZC-54271, China), and rat toll-like receptor 4 (TLR4) ELISA kit (CUSABIO, CSB-E15822r, China).

    Techniques: Immunofluorescence, Western Blot